Summary: A resonant-scanning multiphoton optical microscope (MPM) with a millimeter-scale field-of-view (FOV) often encounters a poor Nyquist figure-of-merit (NFOM). leading to an aliasing effect owing to limited effective voxel-sampling rate. In this protocol. we provide a design guideline to enable high-NFOM MPM imaging while simultaneously securing a large FOV/digital-resolution ra... https://www.shopwildwestes.shop/product-category/feather-imitation-eagle/