An Easy, economical approach for preparing cell lysates appropriate for downstream reverse transcription quantitative PCR To maintain RNA integrity, cells and tissues are first lysed by incubation in a very chaotropic ion lysis buffer Option, which immediately inactivates RNases. This post is currently being created freely accessible through PubMed Central https://www.facebook.com/NewTechFind/posts/pfbid0Zk2FE1nAYoGTFXz5KgvF8kujADW2dEMVtPPMJDwFX3jpPTVwT2G4Sxhb7i4Vdv8Dl